human decorin duoset elisa Search Results


enzyme linked immunosorbent assay elisa  (R&D Systems)
94
R&D Systems enzyme linked immunosorbent assay elisa
Systemic delivery of recombinant decorin into SCID mice injected with MDA-MB-231-HM cells. SCID mice injected with the MDA-MB-231-HM cells into mammary pads received 2 different doses of recombinant decorin systemically. (A) Tumor volume, (B) metastatic burden, (C) plasma decorin levels, and (D) tumor decorin levels after the low-dose experiment. Mice were dosed every second day with 100 µg of recombinant decorin or an equal volume of saline (Control) ∼14 days post tumor-cell inoculation. (E) Tumor volume, (F) metastatic burden, (G) plasma decorin levels, and (H) tumor decorin levels after the high-dose experiment. Mice were dosed daily with 10 mg/kg body weight (∼2.5 times the dose of low-dose experiment) of recombinant decorin or an equal volume of saline (Control) ∼7 days post tumor-cell inoculation. Tumor volume was assessed by calliper measurement of primary tumor growth and calculated as (length × width )/2. Metastatic burden was assessed by bioluminescence imaging detection. Plasma decorin levels were measured by <t>ELISA.</t> Tumor decorin levels were measured by western blot using total protein normalization. Two AAV:Dcn gastrocnemius lysates from the overexpression experiments were used as positive controls. Statistical testing was done with two-way analyses of variance or mixed effects model for repeated measures or Student's t test. Data expressed as means ± SEM. ( n = 5–7 mice per group). A.U. = arbitrary unit; con = control; Dcn = decorin; ELISA = enzyme linked <t>immunosorbent</t> assay.
Enzyme Linked Immunosorbent Assay Elisa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+decorin+duoset+elisa/Human+Decorin+DuoSet+ELISA/pmc11809198-53-10-18
Average 94 stars, based on 1 article reviews
enzyme linked immunosorbent assay elisa - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier
human decorin  (R&D Systems)
94
R&D Systems human decorin
Systemic delivery of recombinant decorin into SCID mice injected with MDA-MB-231-HM cells. SCID mice injected with the MDA-MB-231-HM cells into mammary pads received 2 different doses of recombinant decorin systemically. (A) Tumor volume, (B) metastatic burden, (C) plasma decorin levels, and (D) tumor decorin levels after the low-dose experiment. Mice were dosed every second day with 100 µg of recombinant decorin or an equal volume of saline (Control) ∼14 days post tumor-cell inoculation. (E) Tumor volume, (F) metastatic burden, (G) plasma decorin levels, and (H) tumor decorin levels after the high-dose experiment. Mice were dosed daily with 10 mg/kg body weight (∼2.5 times the dose of low-dose experiment) of recombinant decorin or an equal volume of saline (Control) ∼7 days post tumor-cell inoculation. Tumor volume was assessed by calliper measurement of primary tumor growth and calculated as (length × width )/2. Metastatic burden was assessed by bioluminescence imaging detection. Plasma decorin levels were measured by <t>ELISA.</t> Tumor decorin levels were measured by western blot using total protein normalization. Two AAV:Dcn gastrocnemius lysates from the overexpression experiments were used as positive controls. Statistical testing was done with two-way analyses of variance or mixed effects model for repeated measures or Student's t test. Data expressed as means ± SEM. ( n = 5–7 mice per group). A.U. = arbitrary unit; con = control; Dcn = decorin; ELISA = enzyme linked <t>immunosorbent</t> assay.
Human Decorin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+decorin+duoset+elisa/Human+Decorin+DuoSet+ELISA/pmc06303295-202-12-14
Average 94 stars, based on 1 article reviews
human decorin - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier

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Systemic delivery of recombinant decorin into SCID mice injected with MDA-MB-231-HM cells. SCID mice injected with the MDA-MB-231-HM cells into mammary pads received 2 different doses of recombinant decorin systemically. (A) Tumor volume, (B) metastatic burden, (C) plasma decorin levels, and (D) tumor decorin levels after the low-dose experiment. Mice were dosed every second day with 100 µg of recombinant decorin or an equal volume of saline (Control) ∼14 days post tumor-cell inoculation. (E) Tumor volume, (F) metastatic burden, (G) plasma decorin levels, and (H) tumor decorin levels after the high-dose experiment. Mice were dosed daily with 10 mg/kg body weight (∼2.5 times the dose of low-dose experiment) of recombinant decorin or an equal volume of saline (Control) ∼7 days post tumor-cell inoculation. Tumor volume was assessed by calliper measurement of primary tumor growth and calculated as (length × width )/2. Metastatic burden was assessed by bioluminescence imaging detection. Plasma decorin levels were measured by ELISA. Tumor decorin levels were measured by western blot using total protein normalization. Two AAV:Dcn gastrocnemius lysates from the overexpression experiments were used as positive controls. Statistical testing was done with two-way analyses of variance or mixed effects model for repeated measures or Student's t test. Data expressed as means ± SEM. ( n = 5–7 mice per group). A.U. = arbitrary unit; con = control; Dcn = decorin; ELISA = enzyme linked immunosorbent assay.

Journal: Journal of Sport and Health Science

Article Title: Decorin, an exercise-induced secretory protein, is associated with improved prognosis in breast cancer patients but does not mediate anti-tumorigenic tissue crosstalk in mice

doi: 10.1016/j.jshs.2024.100991

Figure Lengend Snippet: Systemic delivery of recombinant decorin into SCID mice injected with MDA-MB-231-HM cells. SCID mice injected with the MDA-MB-231-HM cells into mammary pads received 2 different doses of recombinant decorin systemically. (A) Tumor volume, (B) metastatic burden, (C) plasma decorin levels, and (D) tumor decorin levels after the low-dose experiment. Mice were dosed every second day with 100 µg of recombinant decorin or an equal volume of saline (Control) ∼14 days post tumor-cell inoculation. (E) Tumor volume, (F) metastatic burden, (G) plasma decorin levels, and (H) tumor decorin levels after the high-dose experiment. Mice were dosed daily with 10 mg/kg body weight (∼2.5 times the dose of low-dose experiment) of recombinant decorin or an equal volume of saline (Control) ∼7 days post tumor-cell inoculation. Tumor volume was assessed by calliper measurement of primary tumor growth and calculated as (length × width )/2. Metastatic burden was assessed by bioluminescence imaging detection. Plasma decorin levels were measured by ELISA. Tumor decorin levels were measured by western blot using total protein normalization. Two AAV:Dcn gastrocnemius lysates from the overexpression experiments were used as positive controls. Statistical testing was done with two-way analyses of variance or mixed effects model for repeated measures or Student's t test. Data expressed as means ± SEM. ( n = 5–7 mice per group). A.U. = arbitrary unit; con = control; Dcn = decorin; ELISA = enzyme linked immunosorbent assay.

Article Snippet: The concentration of decorin was measured in EV-free plasma with enzyme linked immunosorbent assay (ELISA) (DuoSet, Cat# DY143; R&D Systems, Minneapolis, MN, USA).

Techniques: Recombinant, Injection, Clinical Proteomics, Saline, Control, Imaging, Enzyme-linked Immunosorbent Assay, Western Blot, Over Expression